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KMID : 1037420160110020053
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Journal of Korean Skull Base Society
2016 Volume.11 No. 2 p.53 ~ p.58
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The Effect of Low Frequency Electromagnetic Fields on the Proliferation of vestibular schwann cells
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Yoon Yeo-Jung
Moon In-Seok
Park Gwan-Soo
Lee Jong-Dae
Song Ki-Won
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Abstract
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Objectives : Authors reported that the time of mobile phone usage can be related with the vestibular schwannoma growth. In this study, we tried to approach to the biological effect of the extremely low frequency electromagnetic field (ELF-EMF) on NF2-/- mouse schwann (SC4) cells.
Experimental Design : We used SC4 cell line and optimized the ELF-EMF exposure conditions with the ELF-EMF exposure results of other cells. For acute response, we exposed 1 and 10mT ELF-EMF to SC4 cells for 30minutes. For continuous exposures, SC4 cell were incubated in the 1 and 10mT ELF-EMF up to 144 hr. Cell viability was measured by MTT assays and proteins were detected by western blots. Distribution of cells in each stage of the cell cycle was analyzed by Fluorescence-activated cell sorting (FACS).
Results : SC4 cells exposed to the continuous as well as the acute ELF-EMF did not show any DNA double strand breaks and apoptosis up to 144 hr. Interestingly, the proliferation of SC4 cells was increased by continuous exposures to ELF-EMF in an intensity and dose dependent manner. However, no activation of Erk and Akt pathways was observed in these cells. Moreover, both the EMF-exposed and unexposed SC4 cells showed similar distribution of cells in each stage of the cell cycle.
Conclusion : These results demonstrated that ELF-EMF of the similar total value of multiplied energy and exposure time of RF-EMF can accelerate the proliferation of SC4, indirectly supporting a positive correlation between the cell phone usage and the tumor size.
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KEYWORD
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vestibular schwannoma, mobile phone, extremely low frequency electromagnetic field
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